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Jenis hepatitis ini disebabkan oleh infeksi virus hepatitis B (HBV). Hepatitis B dapat ditularkan melalui hubungan seksual tanpa alat pengaman dan transfusi darah. Pada kasus yang jarang terjadi, ibu hamil yang terinfeksi virus hepatitis B bisa menularkan virus ini ke janinnya.

Peradangan hati juga bisa terjadi akibat infeksi cacing hati, yaitu opisthorchiidae dan fasciolidae. Salah satu spesies cacing hati jenis opisthorchiidae yang paling sering menyebabkan infeksi adalah Clonorchis.

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